The Department of Cell Biology maintains state-of the art microscopy core facilities to support a wide range biological imaging needs.

BMSB Imaging Core. The main imaging core is located in newly renovated space on the first floor of the Basic Medical Sciences building (Suite 124) dedicated to biological imaging needs. Instrumentation includes:

  • Super-resolution Nikon N-SIM E/STORM microscope. The microscope is built on a Nikon Ti2 inverted fluorescence microscope equipped for Differential Interference Contrast and fluorescence imaging in the blue, green, red, and far red channels at magnifications of 4X, 10X, 20X, and 100X. Super-resolution imaging modalities include TIRF (Total Internal Reflectance Fluorescence), Structured Illumination Microscopy (SIM), and Stochastic Optical Reconstruction Microscopy (STORM). The system is equipped with a stage top incubator for live cell imaging. The system uses Nikon Elements software to control image acquisition and analysis.
  • Super-resolution Nikon CSU-W1/SoRa spinning disk confocal microscope. The microscope is built on a Nikon Ti2 inverted fluorescence microscope equipped for Differential Interference Contrast and fluorescence imaging in the blue, green, red, and far red channels at magnifications of 4X, 10X, 20X, 40X, 60X, and 100X.  The SoRa attachment enables superresolution imaging and the spinning disk design permits confocal imaging through specimens up to 200-300 micrometers thick. The system is equipped with a stage top incubator for live cell imaging, and a fast galvo scanner for dynamic imaging methods including, FRAP, photoconversion, and photostimulation. (Expected delivery in August 2021)
  • Nikon Ti2 Live-Cell Imaging Microscope. The microscope is built on a Nikon Ti2 inverted fluorescence microscope equipped for Differential Interference Contrast and fluorescence imaging in the blue, green, red, and far red channels at magnifications of 4X-100X. The system is designed for imaging of multiple regions of interest within the sample and for image stitching of large fields. The system is equipped with a stage-top incubator that accepts standard lab culture ware for long-term experiments. The system is configured with two illumination decks to support different types of imaging needs. For standard live cell imaging needs, the system is equipped with an LED illumination system. For short-term experiments requiring dynamic imaging of activity-dependent fluorescent dyes (such as Fura-2, Fluo4) or genetically-encoded biosensors (such as GCaMP or synaptopHluorin), a high intensity Xenon light source is available. The system uses Nikon Elements software to control image acquisition and analysis.
  • Leica M205-MFC THUNDER microscope for 3D and 2D imaging of large specimens at magnifications from 1X-40X. Supports imaging in blue, green, near red and far red fluorescence channels or white light. The system is designed for imaging of multiple regions of interest within the sample and for image stitching of large fields in 2D and 3D. Leica LasX software is used for image acquisition and for THUNDER computational clearing of out-of-focus light in fluorescently labeled specimens.
  • Epi-fluorescence microscopes. Nikon TsR and T2000 Eclipse microscopes capable of phase contrast and fluorescence imaging in the blue, green, red and far red channels at 4X-60X are available.
  • Brightfield microscopes. Upright and inverted microscopes for observation of histologically stained samples at 4X-40X.
  • Stereomicroscope. A stereomicroscope is available for observation of large specimens at low magnifications.

BRC Imaging Core

  • Olympus Fluoview 1000 Laser scanning confocal microscope. This microscope is equipped for confocal imaging at 4X-100X in the blue, green, near red, and far red fluorescence channels. Image acquisition is controlled by Olympus Fluoview software.
Olympus Fluoview 1000 Multi-photon microscope.