Imaging Core

Light Microscopy Facilities Available in the Biomedical Sciences Building (BMSB)

Imaging and Preparative Facilities available through the Department of Cell Biology Imaging Core:

The Cell Biology Imaging Core provides access to a wide range of imaging and tissue preparation equipment and facilities.

Tissue Preparation: Facilities and equipment for preparation and sectioning of frozen and paraffin-embedded tissue sections are available via histology cores in the Stephenson Cancer Center, Harold Hamm Diabetes Center (HHDC), and Dean McGee Eye Institute (DMEI). Several cryostats and paraffin microtomes are available.

Microscopes in the Cell Biology Imaging Core: The Cell Biology Imaging Core provides access to a range of light microscopes for brightfield and fluorescence imaging at magnifications from 1X-100X. Epifluorescence Microscopes: Nikon Eclipse and Olympus IX81 epifluorescence microscopes equipped for three or four channel fluorescence imaging at 4x-100x. Live Cell Imaging: Nikon Ti2 multifluorescence live cell imaging microscope for long-term imaging and for live cell dye imaging applications. 2D/3D reconstruction: Leica M205-MFC 2D/3D imaging system with THUNDER computational clearing equipped for four channel fluorescence and brightfield imaging, and 2D/3D reconstruction of large specimens. Confocal microscopy: Olympus Fluoview 1000 laser scanning confocal microscope equipped with six laser lines. Multiphoton Microscopy: Olympus Fluoview 1000 Multiphoton microscope.

Automated Slide Scanner: An Olympus VS120 virtual slide scanner equipped for automated slide scanning in brightfield and three channel fluorescent modes at magnifications of 4x-100x via the HHDC.

Laser Capture Microdissection: A Leica LMD7 laser capture microdissection system is available without charge through the OUHSC Laser Capture Microdissection core. This state-of-the-art system is equipped for laser capture microdissection at magnifications from 4x-100x with fixed or unfixed tissue preparations.

Super-Resolution Microscopy: A DeltaVision OMX-SR super resolution microscopy system capable of highspeed 2D and 3D Structured Illumination Microscopy (SIM), Ring-TIRF, widefield, and localization microscopy is available for an hourly fee through the Oklahoma Medical Research Foundation (OMRF) Imaging Facility located adjacent to the OUHSC campus.

Transmission Electron Microscopy: An Hitachi H-7600 transmission electron microscope, equipped for standard and cryo-EM applications is available for an hourly fee through the OMRF Imaging Facility, located adjacent to the OUHSC campus.

Other biological imaging equipment available on campus: The OUHSC Flow Cytometry and Imaging Core provides state-of-the-art microscopy, cell analysis and sorting instrumentation on a fee-for use basis. Available equipment includes: state-of-the-art Leica TCS SP8 and Leica SP2 MP multiphoton laser scanning confocal microscopes for high resolution confocal imaging needs; Nikon TE2000E inverted epifluorescent microscope with CO2 chamber for live cell imaging; FACSCalibur four color automated cell-sorting system with dual laser excitation (488 and 633nm) and automated sample loading; Influx cell sorter with 4 laser excitation (UV, 405, 488 and 633nm) for cell sorting and kinetic measurements. FACSJazz cell sorter set up for sorting BSL2 samples with 3 laser excitation lines (405, 488, 640 nm); Stratedigm S1200Ex flow cytometer (3 lasers up to 13 colors), CellCapTure acquisition and analysis software, A600 plate loader, and workstation; BioPlex 200 high-throughput multiplex system for: protein expression, cell signaling, genotyping, autoimmune, cystic fibrosis, cancer, cardiac, metabolic markers, and HLA testing (configured to allow users to create up to 100-plex per microtiter well). The facility also maintains a sterile hood, CO2 incubator, centrifuge, and fluorescent microscope for cell preparation and tissue culture work.

For technical questions related to the Cell Biology Light Microscopy Core please contact: 

Dr. David Sherry

Office Phone:  405-271-8000  ext. 45514
Office Location: BMSB 536a
E-mail:  David-Sherry@ouhsc.edu

University of Oklahoma Health Sciences Center
940 S L Young Blvd
Oklahoma City, OK 73104

 

 

Nikon Ti2-E - Live Cell Imaging System

 
Location:  BMSB 519

Nikon Ti2-E - Small


Specifications:     

  • 4X to 100X (Oil) objectives, 
  • Epifluorescence (Blue, Green, Near Red and Deep Red)
  • Phase contrast
  • Encoded Motorized X-Y Stage
  • Stage Top Incubator with 35 mm Dish, Chamber Slide, multi-well plate Inserts
  • Tokai Hit TID-NA Motorized Stage Adapter
  • Zyla 4.2 Plus sCMOS camera

Features:

  • Live Cell Imaging
  • 2D imaging
  • 3D imaging
  • Mosaic stitching
  • High-speed data acquisition
  • Deconvolution
  • Live cell functional dye imaging
  • Hardware for heating and cooling imaging using open-design recording chambers.  

    Workstation:

  • Nikon Elements

 

 

Nikon TE 2000 Eclipse inverted epifluorescence microscope

 

Location: BMSB 519

  

Nikon T200smallest

  

 Specifications:

  • LED illumination
  • Bright field
  • Phase contrast
  • 4 channel epifluorescence (Blue, Green, Near Red, Far Red)
  • 4X-100X objective lenses
  • 16 bit Andor Zyla sCMOS camera

 Features:

  • 2D Brightfield, Phase contrast, and Epifluorescence imaging

 Workstation:

  • Nikon Elements Software for data acquisition

  

 

Nikon Ts2R-FL Eclipse inverted epifluorescence microscope

 

Location: BMSB 519
   

Ts2R smallest636997748496972735

 

Specifications:

  • LED illumination
  • Bright field
  • Phase contrast
  • 3 Channel epifluorescence (Blue, Green, Near Red)
  • 2X-40X objective lenses
  • Dyno sCMOS camera

Features:

  • 2D Brightfield, Phase contrast, and Epifluorescence imaging

Workstation:

  • Nikon Elements Software for data acquisition

 

 

 

Olympus IX51 compound microscope for histological imaging

 

Location: BMSB 519

Olympus Ix51 smallest


Specifications:

  • Bright field
  • Phase contrast
  • 4X-40X objective lenses
  • Moticam 1.3 megapixel color/BW CMOS camera

Features:

  • 2D Brightfield, Phase contrast, and Epifluorescence imaging

Workstation:

  • Nikon Elements Software for data acquisition

 

 

Leica M205FCA-THUNDER stereomicroscopy system  (Acquisition in progress)

 

Location: BMSB 519 (Once Acquired)

Specifications:

  • Bright field illumination
  • 4 channel epifluorescence (Blue, Green, Near Red, Far Red)
  • 1X -2X objective lenses (7.8X - 160X final magnification)
  • Motorized XYZ stage
  • Leica DFC9000GT sCMOS camera

Features:

  • 2D/3D Brightfield, Phase contrast, and Epifluorescence imaging
  • 2D/3D Image stitching
  • Imaging of large and thick specimens (brain slices, tissue whole mounts, organoids, embryos, etc.)
  • 3D reconstruction of large specimens
  • Computational clearing of fluorescently labeled specimens without chemical tissue clearing.

Workstation:

  • Leica LAS X software for image acquisition and reconstruction.
  • Leica THUNDER software for computational clearing of thick specimens

 

Imaging Core Facilities Available in the Biomedical Research Center (BRC)

Use of the laser scanning microscopes is available to members of the Department of Cell Biology, Harold Hamm Diabetes Center, and the Diabetes NIH CoBRE. User fees are charged monthly to a chart field provided by the user. The current fee structures are:

Olympus FV1000 Laser Scanning Confocal Microscope:

    • $12.53/hr unassisted
    • $20.49/hr assisted


Olympus FV1000MPE Multiphoton Laser Scanning Microscope:

    • $12.55/hr unassisted
    • $20.16/hr assisted


For technical questions related to the Cell Biology Imaging Core in BRC please contact:

Dr. Jody Summers

Office Phone:  405-271-8000  ext. 46478
Office Location: BRC 266
E-mail: jody-summers@ouhsc.edu

University of Oklahoma Health Sciences Center
940 S L Young Blvd
Oklahoma City, OK 73104

 

 
Olympus FV1000 - Laser Scanning Confocal Microscope

 

Location: BRC 271

  

Olympus FV1000

  

Specifications:

  • 10x to 100x objectives (dry, water and oil immersion) 
  • Epifluorescence (Blue, Green, Yellow and Red) 
  • Brightfield (Phase optics, Nomarski DIC) 
  • Automated xyz stage 
  • Universal specimen holder (slides, petri dishes..) 
  • Lasers (405, 458, 488, 515, 559 and 635 nm)
  • Integrated Photomultiplier detector

 Features:   

  • x-y scan (2D)
  • Z-stack (3D)
  • FRET
  • Mosaic Stitching
  • Colocalization
  • Spectral Imaging

 Workstation:  

  • Olympus Fluoview (Version2.1a), Adobe Photoshop, FIJI, Volocity
Olympus FV1000MPE - Multiphoton Laser Scanning Microscope

 

Location: BRC 268

 

Olympus FV1000MPE

 

Specifications:

  • 25x water immersion objective and Interchangeable objectives 10x to 100x (dry, water and oil immersion)
  • Epifluorescence (Blue, Green and Red)
  • Mai Tai DeepSee tunable High Power Ultrafast Lasers (690~1040nm)
  • Non-Descanned Detector

 Features:

  • x-y scan (2D)
  • Z-stack (3D)
  • Fixed and in vivo live samples scan

Workstation:

  • Olympus Fluoview (Version 2.1a), Adobe Photoshop, FIJI, Volocity

 

 

Olympus MVX10 - Research Macro Zoom System Microscope

 

Location: BRC 261

  

Olympus MVX10 - small

 

Specifications:

  • 0.63X to 6.3X (continuous Zoom)
  • Epifluorescence (Blue, Green and Red)
  • DP71 Olympus Digital Camera

Functions:

  • 2D Brightfield and fluorescence imaging
  • in vivo, whole mount sample

Workstation:

  • Image Pro Plus (Version 6.3)
  • Auto Quant X (Version 2.1)

 

 

Olympus Provis AX-70  - Photomicroscope

 

Location: BRC 261

  

Olympus Provis AX-70 - small636584664751610182

 

 Specifications:

  • 4X to 100X objectives (4X, 10X, 20X, 40X and 100X (Oil))
  • Epifluorescence (Blue, Green, and Red)
  • Phase optics
  • Nomarski DIC
  • Q-imaging digital camera

Features:

  • 2D Brightfield and fluorescence imaging
  • Fixed sample

Workstation:

  • Q-capture
  • Auto Quant X (Version 2.1)