Imaging and Preparative Facilities available through the Department of Cell Biology Imaging Core:
The Cell Biology Imaging Core provides access to a wide range of imaging and tissue preparation equipment and facilities.
Tissue Preparation: Facilities and equipment for preparation and sectioning of frozen and paraffin-embedded tissue sections are available via histology cores in the Stephenson Cancer Center, Harold Hamm Diabetes Center (HHDC), and Dean McGee Eye Institute (DMEI). Several cryostats and paraffin microtomes are available.
Microscopes in the Cell Biology Imaging Core: The Cell Biology Imaging Core provides access to a range of light microscopes for brightfield and fluorescence imaging at magnifications from 1X-100X. Epifluorescence Microscopes: Nikon Eclipse and Olympus IX81 epifluorescence microscopes equipped for three or four channel fluorescence imaging at 4x-100x. Live Cell Imaging: Nikon Ti2 multifluorescence live cell imaging microscope for long-term imaging and for live cell dye imaging applications. 2D/3D reconstruction: Leica M205-MFC 2D/3D imaging system with THUNDER computational clearing equipped for four channel fluorescence and brightfield imaging, and 2D/3D reconstruction of large specimens. Confocal microscopy: Olympus Fluoview 1000 laser scanning confocal microscope equipped with six laser lines. Multiphoton Microscopy: Olympus Fluoview 1000 Multiphoton microscope.
Automated Slide Scanner: An Olympus VS120 virtual slide scanner equipped for automated slide scanning in brightfield and three channel fluorescent modes at magnifications of 4x-100x via the HHDC.
Laser Capture Microdissection: A Leica LMD7 laser capture microdissection system is available without charge through the OUHSC Laser Capture Microdissection core. This state-of-the-art system is equipped for laser capture microdissection at magnifications from 4x-100x with fixed or unfixed tissue preparations.
Super-Resolution Microscopy: A DeltaVision OMX-SR super resolution microscopy system capable of highspeed 2D and 3D Structured Illumination Microscopy (SIM), Ring-TIRF, widefield, and localization microscopy is available for an hourly fee through the Oklahoma Medical Research Foundation (OMRF) Imaging Facility located adjacent to the OUHSC campus.
Transmission Electron Microscopy: An Hitachi H-7600 transmission electron microscope, equipped for standard and cryo-EM applications is available for an hourly fee through the OMRF Imaging Facility, located adjacent to the OUHSC campus.
Other biological imaging equipment available on campus: The OUHSC Flow Cytometry and Imaging Core provides state-of-the-art microscopy, cell analysis and sorting instrumentation on a fee-for use basis. Available equipment includes: state-of-the-art Leica TCS SP8 and Leica SP2 MP multiphoton laser scanning confocal microscopes for high resolution confocal imaging needs; Nikon TE2000E inverted epifluorescent microscope with CO2 chamber for live cell imaging; FACSCalibur four color automated cell-sorting system with dual laser excitation (488 and 633nm) and automated sample loading; Influx cell sorter with 4 laser excitation (UV, 405, 488 and 633nm) for cell sorting and kinetic measurements. FACSJazz cell sorter set up for sorting BSL2 samples with 3 laser excitation lines (405, 488, 640 nm); Stratedigm S1200Ex flow cytometer (3 lasers up to 13 colors), CellCapTure acquisition and analysis software, A600 plate loader, and workstation; BioPlex 200 high-throughput multiplex system for: protein expression, cell signaling, genotyping, autoimmune, cystic fibrosis, cancer, cardiac, metabolic markers, and HLA testing (configured to allow users to create up to 100-plex per microtiter well). The facility also maintains a sterile hood, CO2 incubator, centrifuge, and fluorescent microscope for cell preparation and tissue culture work.