Imaging Core

For technical questions related to the Cell Biology Light Microscopy Core please contact: 

Dr. David Sherry

Office Phone:  405-271-8000  ext. 45514
Office Location: BMSB 536a
E-mail:  David-Sherry@ouhsc.edu

University of Oklahoma Health Sciences Center
940 S L Young Blvd
Oklahoma City, OK 73104

 
Location:  BMSB 519

Specifications:     

• 4X to 100X (Oil) objectives, 
• Epifluorescence (Blue, Green, Near Red and Deep Red)
• Phase contrast
• Encoded Motorized X-Y Stage
• Stage Top Incubator with 35 mm Dish, Chamber Slide, multi-well plate Inserts
• Tokai Hit TID-NA Motorized Stage Adapter
• Zyla 4.2 Plus sCMOS camera

• Live Cell Imaging
• 2D imaging
• 3D imaging
• Mosaic stitching
• High-speed data acquisition
• Deconvolution
• Live cell functional dye imaging
• Hardware for heating and cooling imaging using open-design recording chambers.  

    Workstation:

• Nikon Elements

Location: BMSB 519

 Specifications:

• LED illumination
• Bright field
• Phase contrast
• 4 channel epifluorescence (Blue, Green, Near Red, Far Red)
• 4X-100X objective lenses
• 16 bit Andor Zyla sCMOS camera

 Features:

• 2D Brightfield, Phase contrast, and Epifluorescence imaging

 Workstation:

• Nikon Elements Software for data acquisition

Location: BMSB 519
   

Specifications:

• LED illumination
• Bright field
• Phase contrast
• 3 Channel epifluorescence (Blue, Green, Near Red)
• 2X-40X objective lenses
• Dyno sCMOS camera

Features:

• 2D Brightfield, Phase contrast, and Epifluorescence imaging

Workstation:

• Nikon Elements Software for data acquisition

Location: BMSB 519

Specifications:

• Bright field
• Phase contrast
• 4X-40X objective lenses
• Moticam 1.3 megapixel color/BW CMOS camera

Features:

• 2D Brightfield, Phase contrast, and Epifluorescence imaging

Workstation:

• Nikon Elements Software for data acquisition

Location: BMSB 519 (Once Acquired)

Specifications:

• Bright field illumination
• 4 channel epifluorescence (Blue, Green, Near Red, Far Red)
• 1X -2X objective lenses (7.8X - 160X final magnification)
• Motorized XYZ stage
• Leica DFC9000GT sCMOS camera

Features:

• 2D/3D Brightfield, Phase contrast, and Epifluorescence imaging
• 2D/3D Image stitching
• Imaging of large and thick specimens (brain slices, tissue whole mounts, organoids, embryos, etc.)
• 3D reconstruction of large specimens
• Computational clearing of fluorescently labeled specimens without chemical tissue clearing.

Workstation:

• Leica LAS X software for image acquisition and reconstruction.
• Leica THUNDER software for computational clearing of thick specimens

Use of the laser scanning microscopes is available to members of the Department of Cell Biology, Harold Hamm Diabetes Center, and the Diabetes NIH CoBRE. User fees are charged monthly to a chart field provided by the user. The current fee structures are:

Olympus FV1000 Laser Scanning Confocal Microscope:

• $12.53/hr unassisted
• $20.49/hr assisted

Olympus FV1000MPE Multiphoton Laser Scanning Microscope:

• $12.55/hr unassisted
• $20.16/hr assisted

For technical questions related to the Cell Biology Imaging Core in BRC please contact:

Dr. Jody Summers

Office Phone:  405-271-8000  ext. 46478
Office Location: BRC 266
E-mail: jody-summers@ouhsc.edu

University of Oklahoma Health Sciences Center
940 S L Young Blvd
Oklahoma City, OK 73104

Location: BRC 271

Specifications:

• 10x to 100x objectives (dry, water and oil immersion) 
• Epifluorescence (Blue, Green, Yellow and Red) 
• Brightfield (Phase optics, Nomarski DIC) 
• Automated xyz stage 
• Universal specimen holder (slides, petri dishes..) 
• Lasers (405, 458, 488, 515, 559 and 635 nm)
• Integrated Photomultiplier detector

 Features:   

• x-y scan (2D)
• Z-stack (3D)
• FRET
• Mosaic Stitching
• Colocalization
• Spectral Imaging

 Workstation:  

• Olympus Fluoview (Version2.1a), Adobe Photoshop, FIJI, Volocity

Location: BRC 268

Specifications:

• 25x water immersion objective and Interchangeable objectives 10x to 100x (dry, water and oil immersion)
• Epifluorescence (Blue, Green and Red)
• Mai Tai DeepSee tunable High Power Ultrafast Lasers (690~1040nm)
• Non-Descanned Detector

 Features:

• x-y scan (2D)
• Z-stack (3D)
• Fixed and in vivo live samples scan

Workstation:

• Olympus Fluoview (Version 2.1a), Adobe Photoshop, FIJI, Volocity

Location: BRC 261

Specifications:

• 0.63X to 6.3X (continuous Zoom)
• Epifluorescence (Blue, Green and Red)
• DP71 Olympus Digital Camera

Functions:

• 2D Brightfield and fluorescence imaging
• in vivo, whole mount sample

Workstation:

• Image Pro Plus (Version 6.3)
• Auto Quant X (Version 2.1)

Location: BRC 261

 Specifications:

• 4X to 100X objectives (4X, 10X, 20X, 40X and 100X (Oil))
• Epifluorescence (Blue, Green, and Red)
• Phase optics
• Nomarski DIC
• Q-imaging digital camera

Features:

• 2D Brightfield and fluorescence imaging
• Fixed sample

Workstation:

• Q-capture
• Auto Quant X (Version 2.1)